{"product_id":"cfebcdspce","title":"Compartmentalized Dual Screen-Printed Carbon Electrode as Flexible Electrochemical Biosensors, CFEBCDSPCE","description":"\u003cp\u003eA Compartmentalized Dual Screen-Printed Carbon Electrode (Compartmentalized Dual-SPCE) solves the primary manufacturing bottleneck of dual-electrode biosensors: cross-contamination during localized surface functionalization. By physically or chemically isolating two adjacent working electrodes (WE1 and WE2) during the drop-casting or immobilization step, distinct enzymes, antibodies, or nanomaterials can be deposited independently without droplet coalescence. During testing, the barrier allows a single sample droplet (30–60 uL) or a continuous flow stream to bridge both working electrodes along with the shared counter (CE) and reference (RE) electrodes.\u003c\/p\u003e\n\u003cp\u003eFor Chip Layout and Electrical Topography: (1) \u003cstrong\u003eIndependent Working Electrodes (WE1 \u0026amp; WE2)\u003c\/strong\u003e: Semicircular, rectangular microband, or disk geometry (Ф 1.5–2.5 mm). Centered within isolated wells separated by a non-conductive barrier band (≥ 0.8–1.2 mm width). (2) \u003cstrong\u003eShared Counter Electrode (CE)\u003c\/strong\u003e: High-surface-area carbon band enclosing the working electrode perimeter (A_CE ≥ 3 * [A_WE1 + A_WE2). (3)\u003cstrong\u003e Shared Reference Electrode (RE)\u003c\/strong\u003e: Screen-printed Ag\/AgCl track providing an identical potentiostatic reference for both channels. (4) \u003cstrong\u003eConnection Interface\u003c\/strong\u003e: 4-pin or 4-track edge card connector interface (WE1, WE2, CE, RE) configured for standard boxed bipotentiostat connectors.\u003c\/p\u003e\n\u003cp\u003eThe Step-by-Step Sensor Fabrication Workflow: (1) \u003cstrong\u003eElectrochemical Pre-Activation\u003c\/strong\u003e: Immerse the entire sensing area in 0.1 M H2SO4 and run cyclic voltammetry sweeps (-0.2 V to +1.2 V vs. Ag\/AgCl at 100 mV\/s for 10 cycles) to strip residual ink binders and generate carboxyl (-COOH) and hydroxyl (-OH) anchoring sites on both carbon disks. (2) \u003cstrong\u003eCompartmentalized Dispensing\u003c\/strong\u003e: \u003cem\u003eWell 1 (Target Channel)\u003c\/em\u003e: Dispense 0.8–1.5 uL of target probe solution (e.g., anti-Troponin I antibody + EDC\/NHS coupling mix). \u003cem\u003eWell 2 (Reference\/Interference Channel)\u003c\/em\u003e: Dispense 0.8–1.5 uL of control solution (e.g., non-specific IgG, BSA, or secondary target enzyme such as Lactate Oxidase). Incubate in a humid chamber (4°C or 37°C) for 1–2 hours. The isolation barrier keeps the droplets mechanically separated. (3) \u003cstrong\u003eWashing \u0026amp; Global Surface Blocking\u003c\/strong\u003e: Aspirate the micro-droplets using an automatic pipette tip directed away from the carbon center. Rinse with PBST (0.05% Tween-20 in PBS). Flood the entire chip well with 50 uL of 1wt% BSA or casein solution for 30 minutes to block unreacted sites across the whole sensing area, then rinse and dry under a gentle N2 stream. (4) \u003cstrong\u003eElectrochemical Interrogation\u003c\/strong\u003e: Apply a single 40–60 uL drop of whole blood, serum, or running buffer bridging all four electrodes. Connect to a bipotentiostat for concurrent differential pulse voltammetry (DPV) or chronoamperometric reading.\u003c\/p\u003e\n\u003ctable border=\"1\" style=\"width: 100.071%; height: 241.4px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 47.6px;\"\u003e\n\u003ctd style=\"width: 24.8016%; height: 47.6px;\"\u003ePart Number\u003c\/td\u003e\n\u003ctd style=\"width: 74.9846%; height: 47.6px;\"\u003e\n\u003cul\u003e\n\u003cli\u003eCFEBCDSPCE (C-FEB-CDSPCE)\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 146.2px;\"\u003e\n\u003ctd style=\"width: 24.8016%; height: 146.2px;\"\u003eCritical Features\u003c\/td\u003e\n\u003ctd style=\"width: 74.9846%; height: 146.2px;\"\u003e\n\u003cul\u003e\n\u003cli\u003eSubstrate Material: PET (thickness=0.28 mm)\u003c\/li\u003e\n\u003cli\u003eWorking Electrode (WE1\/WE2): Carbon Film (Φ 3mm) with physical microwells to avoid cross-contamination during localized surface functionalization.\u003c\/li\u003e\n\u003cli\u003eCounter Electrode (CE): Carbon\u003c\/li\u003e\n\u003cli\u003eReference Electrode (RE): Ag\/AgCl\u003c\/li\u003e\n\u003cli\u003eAn adapter is included for operation. \u003c\/li\u003e\n\u003cli\u003eStorage Conditions: RT, avoid light illumination, Al bags sealing, ~50%RH \u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"height: 47.6px;\"\u003e\n\u003ctd style=\"width: 24.8016%; height: 47.6px;\"\u003ePackage Grade\u003c\/td\u003e\n\u003ctd style=\"width: 74.9846%; height: 47.6px;\"\u003e\n\u003cul\u003e\n\u003cli\u003e25 pcs\/pack\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReferences\u003c\/strong\u003e:\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/pubs.rsc.org\/ay\/article-abstract\/12\/12\/1547\/632819\/Applications-of-electrochemical-sensors-and\"\u003eH. Beitollahi, et al., Applications of electrochemical sensors and biosensors based on modified screen-printed electrodes: a review, Anal. Methods (2020) 12 (12): 1547–1560.\u003c\/a\u003e\u003c\/li\u003e\n\u003cli\u003e\u003ca href=\"https:\/\/www.mdpi.com\/1424-8220\/16\/10\/1761\"\u003eKeiichiro Yamanaka, et al. Printable Electrochemical Biosensors: A Focus on Screen-Printed Electrodes and Their Application, Analytical and Bioanalytical Chemistry, 2005, 382, 884–886.\u003c\/a\u003e\u003c\/li\u003e\n\u003c\/ol\u003e","brand":"HYKJ","offers":[{"title":"Default Title","offer_id":48291071590630,"sku":"CFEBCDSPCE","price":249.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0774\/6591\/1526\/files\/CFEBCDSPCE_main.jpg?v=1788674903","url":"https:\/\/echemsupplies.com\/products\/cfebcdspce","provider":"EChem Supplies","version":"1.0","type":"link"}